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DNA sequence at the end of IS1 required for transposition

โœ Scribed by Gamas, Pascal; Galas, David; Chandler, Michael

Book ID
109741398
Publisher
Nature Publishing Group
Year
1985
Tongue
English
Weight
330 KB
Volume
317
Category
Article
ISSN
0028-0836

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DNA sequences required for translational
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The transposase encoded by insertion sequence IS1 is produced from two out-of-phase reading frames (insA and B'-insB) by translational frameshifting, which occurs within a run of six adenines in the -1 direction. To determine the sequence essential for frameshifting, substitution mutations were intr

The tnpR gene product of TnA is required
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A mutant of TnA no longer recognizing immune plasmids had been isolated. The mutation is complemented in trans by a functional tnpR gene. The requirement for wild type tnpR gene product for the establishment of transposition immunity was confirmed by the use of a derivative of transposon Tn3 in whic