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DNA sequences at the ends of transposon Tn5 required for transposition

โœ Scribed by Johnson, Reid C.; Reznikoff, William S.


Book ID
109731552
Publisher
Nature Publishing Group
Year
1983
Tongue
English
Weight
398 KB
Volume
304
Category
Article
ISSN
0028-0836

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The requirement for both DNA polymerase
โœ Sasakawa, Chihiro ;Uno, Yuko ;Yoshikawa, Masanosuke ๐Ÿ“‚ Article ๐Ÿ“… 1981 ๐Ÿ› Springer ๐ŸŒ English โš– 615 KB

By assaying transposition of Tn5 from lambda b221 cI857 rex::Tn5 (Berg 1977) in polA-proficient and deficient cells, both the polymerase activity and 5' to 3' exonuclease activity of DNA polymerase I have been shown to be required for transposition. This requirement could not be observed in three ot