✦ LIBER ✦

The tnpR gene product of TnA is required for transposition immunity

✍ Scribed by Wallace, L. J. ;Ward, J. M. ;Richmond, M. H.

Publisher: Springer
Year: 1981
Tongue: English
Weight: 543 KB
Volume: 184
Category: Article
ISSN: 0026-8925
DOI: 10.1007/bf00271200

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✦ Synopsis

A mutant of TnA no longer recognizing immune plasmids had been isolated. The mutation is complemented in trans by a functional tnpR gene. The requirement for wild type tnpR gene product for the establishment of transposition immunity was confirmed by the use of a derivative of transposon Tn3 in which both the tnpA and the tnpR genes are partly deleted. This deleted Tn3 was shown to transpose onto an immune plasmid in the presence of a wild type tnpA gene but not in the presence of both tnpA and tnpR genes.

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