The acu-1 gene of Coprinus cinereus is a regulatory gene required for induction of acetate utilisation enzymes

✍ Scribed by Maconochie, Mark K. ;Connerton, Ian F. ;Casselton, Lorna A.

• Publisher: Springer
• Year: 1992
• Tongue: English
• Weight: 895 KB
• Volume: 234
• Category: Article
• ISSN: 0026-8925
• DOI: 10.1007/bf00283841

✦ Synopsis

We have isolated a gene from Coprinus cinereus which cross-hybridises to the facA and acu-5 genes of Aspergillus nidulans and Neurospora crassa, respectively. These genes encode acetyl-CoA synthetase, an enzyme which is inducible by acetate and required for growth on acetate as sole carbon source. We have designated the C. cinereus gene acs-1 and have used transformation to demonstrate its functional homology to the ascomycete genes by complementation of an N. crassa acu-5 mutation. The acs-1 gene has never been identified by mutation; mutations leading to loss of acetyl-CoA synthetase function map to another gene, acu-1. Using Northern analyses we have shown that acu-1 has a regulatory function that is required for acetate-induced transcription of acs-1 and of another acetate utilisation gene, acu-7, the isocitrate lyase structural gene.