DNA-repair characterization of cdc40-1, a cell-cycle mutant of Saccharomyces cerevisiae
โ Scribed by Martin Kupiec; Giora Simchen
- Book ID
- 113193005
- Publisher
- Elsevier Science
- Year
- 1986
- Tongue
- English
- Weight
- 538 KB
- Volume
- 162
- Category
- Article
- ISSN
- 0027-5107
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Using the thermoconditional yeast mutant rev2ts that controls an apparently site-specific step of mutagenic DNA repair it was possible to measure the time course of REV2 dependent UV-induced reversion of the ochre allele his5-2 and recovery of survival for UV-treated stationary phase cells: due to t
The time course of REV2 dependent recovery from prelethal UV damage and UV-induced locus-specific reversion of the his5-2 allele was determined in temperature-shift experiments by use of a thermoconditional allele of the rev2 gene (rad5-8, rev2ts). In UV-irradiated, exponentially growing rev2ts cell