DNA fixation and development of transformability and transfectability in Bacillus subtilis
β Scribed by Oostindier-Braaksma, Fietje ;Epstein, H. T.
- Publisher
- Springer
- Year
- 1970
- Tongue
- English
- Weight
- 288 KB
- Volume
- 108
- Category
- Article
- ISSN
- 0026-8925
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Polyethylene glycol-treated protoplasts of B. subtilis can be transformed by plasmid DNA at very high frequencies (Chang and Cohen 1979). From analysis of plasmid mediated transformation of transformation-deficient mutants it appeared that mutants, reduced in the transformation by plasmid DNA in the
In re-extracted DNA obtained shortly after uptake of transforming DNA by Bacillus subtilis, increased amounts of donor DNA radioactivity banding at the position of donor-recipient DNA complex (DRC) are observed in CsCl gradients, if the cells are irradiated with high doses of UV prior to reextractio
Hybrid plasmids were constructed in which the transcription of regions of inserted DNA was defined. Cells containing these plasmids were transformed with monomeric forms of a different hybrid plasmid, which contained, however, the same inserted DNA as the resident plasmid. The transformation frequen
Transformation of competent cells of Bacillus subtilis with antibiotic resistance plasmid DNA has shown that (a) competence for plasmid and chromosomal DNA develops with similar kinetics; (b) DNA linearized with a variety of restriction endonucleases does not transform; (c) CCC plasmid DNA is inacti