DNA replication during development of competence in Bacillus subtilis
โ Scribed by Barlati, Sergio
- Publisher
- Springer
- Year
- 1972
- Tongue
- English
- Weight
- 367 KB
- Volume
- 118
- Category
- Article
- ISSN
- 0026-8925
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Bacillus subtilis were x-irradiated before and after centrifugal separation of cells in a Renografin density gradient. 1)re-competent cultures have no cells at the radiosensitivity of the cells in the bulk competent culture, but there is a substantial fraction of cells in a multi-target state, with
The initial attachment of transforming DNA to competent Bacillus subtilis is temperature independent between 25 degrees and 45 degrees. However, below 15 degrees there is a significant reduction in the amount of DNA attached to compentent cells. The DNA that is attached at 4 degrees can lead to tran