DNA electrophoretic mobility and deformation in agarose gels
โ Scribed by Benjamin Chu; Zhulun Wang
- Book ID
- 115988211
- Publisher
- Elsevier Science
- Year
- 1991
- Tongue
- English
- Weight
- 565 KB
- Volume
- 131-133
- Category
- Article
- ISSN
- 0022-3093
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๐ SIMILAR VOLUMES
We have investigated several experimental factors which affect the accurate determination of electrophoretic mobilities of circular and linear DNAs in agarose gels. We demonstrate that: (1) The mobility of individual DNA species is affected by the total mass in the sample loaded. The increased mobil
The field free diffusion constant and the electric field dependence of the electrophoretic mobility and molecular orientation of DNA samples from 5 to 164 kilobase pairs in agarose gels from 0.5 to 2% have been measured by fluorescence recovery after photobleaching and birefringence. In conditions w