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Orthogonal electrophoretic fractionation of DNA in agarose gels

โœ Scribed by Daniele Bellavia; Giorgia Sisino; Giada Sisino; Rainer Barbieri


Book ID
116735972
Publisher
Elsevier Science
Year
2008
Tongue
English
Weight
366 KB
Volume
22
Category
Article
ISSN
0890-8508

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The field free diffusion constant and the electric field dependence of the electrophoretic mobility and molecular orientation of DNA samples from 5 to 164 kilobase pairs in agarose gels from 0.5 to 2% have been measured by fluorescence recovery after photobleaching and birefringence. In conditions w