DNA determination of yeast by flow cytometry
โ Scribed by K.J. Hutter; H.E. Eipel
- Book ID
- 109310052
- Publisher
- John Wiley and Sons
- Year
- 1978
- Tongue
- English
- Weight
- 199 KB
- Volume
- 3
- Category
- Article
- ISSN
- 0378-1097
No coin nor oath required. For personal study only.
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Cell division cycle (cdc) mutants of Schizosaccharomyces pombe are arrested at specific points in the cell cycle when grown at restrictive temperature. Flow cytometry of such cells reveals an anomalous increase in the DNA fluorescence signal, which represents a problem in experiments designed to det
The variation of the fluorescence intcnsity of olivomycin-stained yeast cells as a function of the concentrdtion of olivomycin, NaCI, and MgCI, in the staining solution was studied. The best results were obtained when the staining solution contained 100 pg olivomycin/ml, 40 mM MgC12, and 1 M NaCI.