✦ LIBER ✦

DNA deletions in spontaneous chloramphenicol-sensitive mutants of Streptomyces coelicolor A3(2) and Streptomyces lividans 66

✍ Scribed by Flett, Fiona ;Cullum, John

Publisher: Springer
Year: 1987
Tongue: English
Weight: 411 KB
Volume: 207
Category: Article
ISSN: 0026-8925
DOI: 10.1007/bf00331621

No coin nor oath required. For personal study only.

✦ Synopsis

A mutant of Streptomyces coelicolor A 3(2) highly resistant to chloramphenicol was selected. It had amplified some chromosomal DNA fragments to a copy number of 20-50. Some of the amplified fragments were cloned and used as hybridisation probes to investigate the spontaneous chloramphenicol-sensitive mutants which occur at high frequency in this species and the closely related species Streptomyces lividans 66. These investigations demonstrated that chloramphenicol sensitivity in both species is associated with large deletions that are at least 40 kb in length.

📜 SIMILAR VOLUMES

Spontaneous mutations in the galactose o

Spontaneous mutations in the galactose operons of Streptomyces coelicolor A3 (2) and Streptomyces lividans 66

✍ Umaru Ali-Dunkrah; Kevin Kendall; Prof. Dr. John Cullum 📂 Article 📅 1990 🏛 John Wiley and Sons 🌐 English ⚖ 403 KB

## Abstract 17 spontaneous Gal^−^ mutants of __S. coelicolor__ were studied using Southern blottings, but no insertion or deletion mutations were found. 10 mutants of __S. lividans__ included three deletion mutations. The reasons for the lack of insertion mutants are discussed.

afsB stimulates transcription of the act

afsB stimulates transcription of the actinorhodin biosynthetic pathway in Streptomyces coelicolor A3(2) and Streptomyces lividans

✍ Horinouchi, Sueharu ;Malpartida, Francisco ;Hopwood, David A. ;Beppu, Teruhiko 📂 Article 📅 1989 🏛 Springer 🌐 English ⚖ 378 KB

DNA rearrangements associated with insta

DNA rearrangements associated with instability of an arginine gene in Streptomyces coelicolor A3(2)

✍ Fiona Flett; Jacqueline Platt; Dr. J. Cullum 📂 Article 📅 1987 🏛 John Wiley and Sons 🌐 English ⚖ 584 KB

## Streptomyces coelicdor A3( 2 ) gives rise t o spontaneous chloramphenicol sensitive mutants a t a frequency of about 0.3% per spore. These mutants are often genetically unstable and give rise to arginine auxotrophs ( h g -) a t frequencies of 1-7% per spore. These Arg-mutants usually lack the

ChemInform Abstract: Actinoperylone, a N

ChemInform Abstract: Actinoperylone, a Novel Perylenequinone-Type Shunt Product, from a Deletion Mutant of the actVA-ORF5 and ORF6 Genes for Actinorhodin Biosynthesis in Streptomyces coelicolor A3(2).

✍ Takaaki Taguchi; Susumu Okamoto; Takayuki Itoh; Yutaka Ebizuka; Kozo Ochi; Koji 📂 Article 📅 2008 🏛 John Wiley and Sons ⚖ 14 KB 👁 1 views

## Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable v