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DNA damage in exfoliated buccal cells of smokers assessed by the single cell gel electrophoresis assay

✍ Scribed by E. Rojas; M. Valverde; M. Sordo; P. Ostrosky-Wegman


Book ID
116098504
Publisher
Elsevier Science
Year
1996
Tongue
English
Weight
688 KB
Volume
370
Category
Article
ISSN
0165-1218

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The single cell gel electrophoresis (SCGE) assay, or comet assay, is a highly sensitive method to measure DNA damage. The principle of the assay is based on the conversion of single-strand breaks and alkali-labile sites to double-strand breaks via alkaline lysis. The cells are subjected to electroph