Improved single-cell gel electrophoresis assay for detecting DNA damage in Eisenia foetida
✍ Scribed by Walter D. Di Marzio; María E. Saenz; Sebastien Lemière; Paule Vasseur
- Publisher
- John Wiley and Sons
- Year
- 2005
- Tongue
- English
- Weight
- 132 KB
- Volume
- 46
- Category
- Article
- ISSN
- 0893-6692
- DOI
- 10.1002/em.20153
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The single cell gel electrophoresis (SCGE) assay, or comet assay, is a highly sensitive method to measure DNA damage. The principle of the assay is based on the conversion of single-strand breaks and alkali-labile sites to double-strand breaks via alkaline lysis. The cells are subjected to electroph
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