Use of the Single-Cell Gel Electrophoresis Assay for the Immunofluorescent Detection of Specific DNA Damage
โ Scribed by Sylvie Sauvaigo; Christine Serres; Nathalie Signorini; Nathalie Emonet; Marie-Jeanne Richard; Jean Cadet
- Publisher
- Elsevier Science
- Year
- 1998
- Tongue
- English
- Weight
- 208 KB
- Volume
- 259
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
The single cell gel electrophoresis (SCGE) assay, or comet assay, is a highly sensitive method to measure DNA damage. The principle of the assay is based on the conversion of single-strand breaks and alkali-labile sites to double-strand breaks via alkaline lysis. The cells are subjected to electroph
The analysis of the genotoxicity of praziquantel, an effective antihelminthic widely used in countries where parasitic infections are still serious public health problems, has been extensively performed using diverse in vitro and in vivo assays and endpoints. However, results are not conclusive, sin
## Abstract The aim of this study was to investigate the protective effect of isothiocyanates towards Nโnitrosamineโinduced DNA damage in the singleโcell gel electrophoresis (SCGE)/HepG2 assay. None of the isothiocyanates (ITCs) concentrations tested in the presence or absence of formamidopyrimidin