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Evaluation of DNA damage in leukocytes of ex-smokers by single cell gel electrophoresis

✍ Scribed by Giada Frenzilli; Cecilia Betti; Tania Davini; Massimiliano Desideri; Edo Fornai; Liliana Giannessi; Fabrizio Maggiorelli; Paolo Paoletti; Roberto Barale


Book ID
114071215
Publisher
Elsevier Science
Year
1997
Tongue
English
Weight
229 KB
Volume
375
Category
Article
ISSN
0027-5107

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The single cell gel electrophoresis (SCGE) assay, or comet assay, is a highly sensitive method to measure DNA damage. The principle of the assay is based on the conversion of single-strand breaks and alkali-labile sites to double-strand breaks via alkaline lysis. The cells are subjected to electroph