Genomic alterations in renal cell carcinoma were investigated by D N A fingerprinting using the simple repetitive oligonucleotide probe (CAC),. Nine of ten tumors showed somatic changes in the fingerprint pattern compared with constitutional DNA. The most consistent changes were deletions and/or dec
DNA content in renal cell carcinoma with reference to tumor heterogeneity
✍ Scribed by BÖRje Ljungberg; Roger Stenling; GÖRan Roos
- Publisher
- John Wiley and Sons
- Year
- 1985
- Tongue
- English
- Weight
- 620 KB
- Volume
- 56
- Category
- Article
- ISSN
- 0008-543X
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## BACKGROUND. A multiple sampling study was performed on 124 specimens of renal cell carcinomas to analyze the consistency and reliability of DNA measurements. The authors investigated intratumoral DNA heterogeneity and its role as a adverse prognostic factor for disease progression. ## METHODS
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Deoxyribonucleic acid (DNA) content was retrospectively determined by single-cell cytophotometry in primary tumors and corresponding metastases from 32 patients with renal cell carcinoma. In 15 of the primary tumors a diploid/near diploid and in 17 an aneuploid DNA content was found. A diploid/near