DNA Cloning in Bacillus subtilis
β Scribed by Stanislav D. Ehrlich
- Book ID
- 123655006
- Publisher
- National Academy of Sciences
- Year
- 1978
- Tongue
- English
- Weight
- 994 KB
- Volume
- 75
- Category
- Article
- ISSN
- 0027-8424
- DOI
- 10.2307/68015
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Different clones carrying a chromosomal DNA fragment able to transform Bacillus subtilis mutants dnaA13, dnaB19, dnaG5, recG40 and polA42 to a wild-type phenotype were isolated from a library constructed in plasmid pJH101. A lambda recombinant clone carrying a chromosomal fragment able to transform
The difficulty experienced in the shotgun cloning of chromosomal DNA on plasmid vectors in Bacillus subtilis is analyzed and an explanation for this difficulty is offered based on an inherent property of competent cells which imposes a requirement of plasmid multimers in transformation of plasmid-fr