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Molecular cloning of a tetracycline-resistance determinant from Bacillus subtilis chromosomal DNA and its expression in Escherichia coli and B. subtilis

✍ Scribed by Reiko Sakaguchi; Kazuo Shishido


Book ID
119119655
Publisher
Elsevier Science
Year
1988
Tongue
English
Weight
785 KB
Volume
949
Category
Article
ISSN
0167-4781

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Expression of the SPR methyltransferase gene from B. subtilis phage SPR cloned into lambda and SPP1 was studied by analyzing the sensitivity of the hybrid phage DNAs to restriction by the enzymes HaeIII, MspI, and HpaII. The following results were obtained: (1) The genes were expressed both in the h