DNA cloning and sequence analysis of chicken AFLP

✍ Scribed by C. Knorr; H. H. Cheng; J. B. Dodgson

• Publisher: John Wiley and Sons
• Year: 2001
• Tongue: English
• Weight: 64 KB
• Volume: 32
• Category: Article
• ISSN: 0268-9146
• DOI: 10.1046/j.1365-2052.2001.00748.x

✦ Synopsis

Amplified fragment length polymorphisms (AFLP) have been shown to be useful for linkage mapping in chickens and other domestic animals. It is often desirable to convert AFLP bands to sequence‐tagged site (STS) markers, in particular, so that AFLP‐based linkage information can be integrated with recombinant DNA clone‐based maps. Sixteen chicken AFLP bands were excised from gels, re‐amplified, cloned and analysed. All inserts proved to be __Eco__RI‐__Taq__I fragments, which suggests that unlabelled __Taq__I‐__Taq__I AFLP fragments do not amplify well, and therefore do not significantly contaminate AFLP bands. For eight of the AFLP, the cloned fragment was used to probe blots of AFLP reaction fingerprints, confirming that the predominant DNA clone indeed contained the polymorphic fragment. Flanking regions of selected AFLP fragments were isolated using Vectorette cloning. The results obtained suggest that the these chicken AFLP most commonly arise from sequence polymorphism at or near the __Taq__I site.