Distribution of UDP-glucuronyl transferase among cell fractions of rat liver

✍ Scribed by S. E. Nyquist; D. James Morre

• Book ID: 102309160
• Publisher: John Wiley and Sons
• Year: 1971
• Tongue: English
• Weight: 341 KB
• Volume: 78
• Category: Article
• ISSN: 0021-9541
• DOI: 10.1002/jcp.1040780103

✦ Synopsis

Microsomal UDP-glucuronyl transferases (uridine-5'-diphosphateglucuronate glucuronyl transferase, acceptor unspecific, EC 2.4.1.17) function in detoxification of a wide range of aglycons containing phenolic, alcoholic, carboxylic or amino acceptor groups through formation of water-soluble glucuronic acid derivatives. To localize this activity within a specific cell component, purified rough-surfaced endoplasmic reticulum and Golgi apparatus fractions from rat liver were compared with total homogenates. The UDP-glucuronyl transferase specific activity of the Golgi apparatus fractions was less than or approximately equal to that of the total homogenate whereas this activity was concentrated 3-8 fold in the rough-surfaced endoplasmic reticulum fraction depending upon substrate. We conclude that the rough-surfaced endoplasmic reticulum i s the major site of glucuronide formation within the microsome fraction of normal rat livers.