Disk Electrophoresis of Basic Proteins and Peptides on Polyacrylamide Gels
β Scribed by REISFELD, R. A.; LEWIS, U. J.; WILLIAMS, D. E.
- Book ID
- 109622397
- Publisher
- Nature Publishing Group
- Year
- 1962
- Tongue
- English
- Weight
- 389 KB
- Volume
- 195
- Category
- Article
- ISSN
- 0028-0836
- DOI
- 10.1038/195281a0
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High-performance capillary sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) has been developed for the separation and molecular weight (MW) determination of peptides and proteins. In this work, acrylamide was polymerized in fused-silica capillaries of 75 microns I.D. and 10 or 20
Basic Protein and Peptide Protocols offers an excellent collection of reproducible, step-by-step laboratory methods covering three major areas: (1) the quantitation and characterization of proteins, (2) the electrophoretic and blotting procedures used in protein isolation and characterization, and (
filter paper reservoirs, obviating the need for tank im-A semidry electrophoretic transfer method was demersion, and transfer times were reduced to less than veloped for efficient electroblotting of proteins sepa-1 h. Due to its convenience and speed, semidry transfer rated by acid-urea polyacrylami