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Discrimination effects and sensitivity variations in matrix-assisted laser desorption/ionization

✍ Scribed by Francisco M. L. Amado; P. Domingues; M. Graça Santana-Marques; A. J. Ferrer-Correia; K. B. Tomer

Publisher
John Wiley and Sons
Year
1997
Tongue
English
Weight
474 KB
Volume
11
Category
Article
ISSN
0951-4198

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✦ Synopsis

In matrix-assisted laser desorption/ionization (MALDI) the analyte signal produced depends strongly on the analyte and on the sample preparation procedure. Comparing the results obtained with the dried-drop method with the results obtained with a homogeneous sample preparation procedure, discrimination effects, as well as sensitivity variations, are shown to be dependent on the molecular weight of the analysed compounds. The signal obtained in MALDI is shown to be dependent on the hydrophobicity and basicity of the analyte. With the dried-drop method, in particular, evidence of peripheral sample deposition of hydrophilic compounds is shown. These last effects are due to mass transfer caused by differences in surface activities during solvent evaporation, and are known as Marangoni effects.

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