β Scribed by Noah P. Christian; Lori Giver; Andrew D. Ellington; James P. Reilly
No coin nor oath required. For personal study only.
Matrix-assisted laser desorptionhonization mass spectra of deoxyribonucleic acid (DNA) homopolymers were recorded using three different matrices under otherwise identical conditions. The matrices were 3-hydroxypicolinic acid, 6-aza-2-thiothymine, and a mixture of 2,3,4-and 2,4,6-trihydroxyacetophenone. Using a time-offlight mass spectrometer with pulsed ion extraction, considerable fragmentation was observed, Parent-ion yields and fragment-ion identities were compared in the three cases. The influence of iron on DNA mass spectra was also investigated and found to be matrix dependent.
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We have developed a method to quantitatively attach quarternary ammonium fixed charge tags to the 5' or 3'NH 2 ends of DNA using N-hydroxysuccinimidyl ester chemistry. The chemical conditions for tagging were chosen so that tagging takes place exclusively on aliphatic NH 2 groups while base amino gr
Identification of proteins using matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) peptide mass fingerprinting (PMF) is a key technique in proteomics. The method is known to be sensitive as well as amenable to high-throughput operation, but the resulting identifications suffer f