The time-of-flight effect in Fourier transform mass spectrometry (FTMS) with an external ion source was quantified. The time-of-flight profiles of ions of different masses were measured by stepping the time interval between the ionization pulse and the gating of the trapping voltage (T gate ). The o
The Effect of Ammonium Salt and Matrix in the Detection of DNA by Matrix-assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry
✍ Scribed by Y. F. Zhu; N. I. Taranenko; S. L. Allman; S. A. Martin; L. Haff; C. H. Chen
- Publisher
- John Wiley and Sons
- Year
- 1996
- Tongue
- English
- Weight
- 771 KB
- Volume
- 10
- Category
- Article
- ISSN
- 0951-4198
No coin nor oath required. For personal study only.
📜 SIMILAR VOLUMES
The ionization of nucleosides in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry was systematically investigated using adenine (A), thymine (T), guanine (G) and cytosine (C) with several common matrices. Experimental results of the protonation and deprotonation of the ba
A variety of gram-positive and gram-negative intact bacterial cells have been analysed by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) and shown to provide fingerprint mass spectra with discrete peaks being observed over the mass range from 3 to 40 kDa. The spectra show b
An improvement in detectability and stability of DNA analysis by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) using oligonucleotides modified with a neutralized backbone and a fixed single, positive charge was recently reported. The attachment of the pos
## Abstract A prototype matrix‐assisted laser desorption/ionization quadrupole time‐of‐flight (MALDI‐QqTOF) tandem mass spectrometer was used to sequence a series of phosphotyrosine‐, phosphothreonine‐ and phosphoserine‐containing peptides. The high mass resolution and mass accuracy of the instrume
Oligonucleotide sequencing by fragmentation in matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry has been demonstrated. With this sequencing method, the preparation of oligonucleotide ladder products and gel electrophoresis are not required, thus the sequencing