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Discrimination between the red- and far-red-absorbing forms of phytochrome fromAvena sativaL. by monoclonal antibodies

✍ Scribed by B. Thomas; S. E. Penn; G. W. Butcher; G. Galfre

Book ID: 104753630
Publisher: Springer-Verlag
Year: 1984
Tongue: English
Weight: 296 KB
Volume: 160
Category: Article
ISSN: 0032-0935
DOI: 10.1007/bf00393420

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✦ Synopsis

A set of rat monoclonal antibodies (ARC MAC 48 to 52 and 54 to 56), raised to phytochrome from dark-grown seedlings of Arena sativa L. was tested for the ability to discriminate between the red-absorbing (Pr) and far-red-absorbing (Pfr) forms of phytochrome by indirect enzyme-linked immunosorbent assay. MAC 50 bound more strongly to Pfr and MAC 49 and 52 showed preferential binding to Pr from extracts of dark-grown Arena seedlings; MAC 50 also bound more strongly to Pfr from brushite-purified phytochrome. The remainder of the monoclonal antibodies and a rabbit polyclonal antiphytochrome preparation did not discriminate between Pr and Pfr. The results provide evidence for conformational changes in defined regions of the phytochrome apoprotein upon photoconversion.

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