Spectral properties and photoactivities of intermediates of photoconversion from the red-light-absorbing to far-red-light-absorbing form of pea phytochrome
✍ Scribed by Sunao Iwakami; Naoya Yoshizawa; Hiro-o Hamaguchi; Yasunori Inoue; Katsushi Manabe
- Publisher
- Elsevier Science
- Year
- 1996
- Tongue
- English
- Weight
- 537 KB
- Volume
- 33
- Category
- Article
- ISSN
- 1011-1344
No coin nor oath required. For personal study only.
✦ Synopsis
The pathway for the phototransformation of the red-light-absorbing (Pr) to the far-red-light-absorbing (Pfr) form of native phytochrome ~, from pea was investigated by low temperature spectrophotometry. Four intermediates between Pr and Pfr were recognized. The first i i termediate (lumi-R) was stable below -100 °C. The other intermediates and Pfr appeared during warming of lumi-R to -80 °C (meta-PaL -40 °C (meta-Rb'), -20 °C (meta-Rc) and 0 °C (Pfr). Each intermediate (lumi-R, meta-Ra and meta-Rc) and Pr was photointert onvertible as a two-component system at the temperature at which the intermediate had initially appeared. Meta-Rb' is also photoconvertible ~ Pr. Prolonged red light irradiation of meta-Rb' at -40 °C resulted in appearance of another intermediate (meta-Rb).