There is a growing demand for high-throughput methods for analysis of single-nucleotide polymorphic (SNP) positions. Here, we have evaluated a novel sequencing approach, pyrosequencing, for such purposes. Pyrosequencing is a sequencing-by-synthesis method in which a cascade of enzymatic reactions yi
Discovery of single nucleotide polymorphisms and mutations by Pyrosequencing
✍ Scribed by Mostafa Ronaghi; Elahe Elahi
- Publisher
- Hindawi Publishing Corporation
- Year
- 2002
- Tongue
- English
- Weight
- 196 KB
- Volume
- 3
- Category
- Article
- ISSN
- 1531-6912
- DOI
- 10.1002/cfg.132
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## Communicated by Christine Van Broeckhoven Pyrosequencing, a non-electrophoretic method for DNA sequencing, is emerging as a popular platform for analysis of single nucleotide polymorphisms (SNPs). This technology has the advantage of accuracy, ease-of-use, and high flexibility for different app
## For the Mutation Detection 2001 Special Issue Here we present the first large-scale effort at genotyping using a novel sequencing method, Pyrosequencing, as a method for genotyping of single nucleotide polymorphisms (SNPs). Pyrosequencing genotypes were validated through duplicate analysis of 1,