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Identification of CYP2D6 alleles by single nucleotide polymorphism analysis using pyrosequencing

✍ Scribed by Anna-Lena Zackrisson; Bertil Lindblom


Publisher
Springer
Year
2003
Tongue
English
Weight
214 KB
Volume
59
Category
Article
ISSN
0031-6970

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Single-Nucleotide Polymorphism Analysis
✍ Afshin Ahmadian; Baback Gharizadeh; Anna C. Gustafsson; Fredrik Sterky; PΓ₯l NyrΓ© πŸ“‚ Article πŸ“… 2000 πŸ› Elsevier Science 🌐 English βš– 129 KB

There is a growing demand for high-throughput methods for analysis of single-nucleotide polymorphic (SNP) positions. Here, we have evaluated a novel sequencing approach, pyrosequencing, for such purposes. Pyrosequencing is a sequencing-by-synthesis method in which a cascade of enzymatic reactions yi