Directed evolution of proteins for heterologous expression and stability
โ Scribed by Cintia Roodveldt; Amir Aharoni; Dan S Tawfik
- Book ID
- 113882946
- Publisher
- Elsevier Science
- Year
- 2005
- Tongue
- English
- Weight
- 232 KB
- Volume
- 15
- Category
- Article
- ISSN
- 0959-440X
No coin nor oath required. For personal study only.
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## Abstract Because of its eukaryotic nature, simple fermentation requirements, and pliable genetics, there have been many attempts at improving recombinant protein production in __Saccharomyces__ __cerevisiae__. These strategies typically involve altering the expression of a native protein thought
Directed evolution has proven to be a successful strategy for the modiยฎcation of enzyme properties. To date, the preferred experimental procedure has been to apply mutations or crossovers randomly throughout the gene. With the emergence of powerful computational methods, it has become possible to de