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Computationally focusing the directed evolution of proteins

โœ Scribed by Christopher A. Voigt; Stephen L. Mayo; Frances H. Arnold; Zhen-Gang Wang

Book ID
102299547
Publisher
John Wiley and Sons
Year
2001
Tongue
English
Weight
85 KB
Volume
84
Category
Article
ISSN
0730-2312

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โœฆ Synopsis

Directed evolution has proven to be a successful strategy for the modiยฎcation of enzyme properties. To date, the preferred experimental procedure has been to apply mutations or crossovers randomly throughout the gene. With the emergence of powerful computational methods, it has become possible to develop focused combinatorial searches, guided by computer algorithms. Here, we describe several computational methods that have emerged to aid the optimization of mutant libraries, the targeting of speciยฎc residues for mutagenesis, and the design of recombination experiments.

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โœ Huimin Zhao ๐Ÿ“‚ Article ๐Ÿ“… 2007 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 150 KB ๐Ÿ‘ 2 views

## Abstract Directed evolution has been successfully used to engineer proteins for basic and applied biological research. However, engineering of novel protein functions by directed evolution remains an overwhelming challenge. This challenge may come from the fact that multiple simultaneously or sy