Directed evolution has become a powerful tool for developing enzyme and whole cell based biocatalysts. Significant recent advances include the creation of novel enzyme functions and the development of several new efficient directed evolution methods. The combination of directed evolution and rationa
Directed evolution of industrial enzymes: an update
β Scribed by Joel R Cherry; Ana L Fidantsef
- Publisher
- Elsevier Science
- Year
- 2003
- Tongue
- English
- Weight
- 128 KB
- Volume
- 14
- Category
- Article
- ISSN
- 0958-1669
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On the way to a combinatorial biotechnology? The directed evolution of enzymes promises a rapid access to effective biocatalysts. New molecular biology techniques for random mutagenesis in combination with high-throughput screening might revolutionize the creation of enzymes with new and improved pr
In the past year, a number of oxidative enzymes have been the target of directed evolution. Catalase reaction specificity has been shifted to peroxidase, the high pH, thermal and oxidative stability of a fungal peroxidase has been dramatically improved, and the substrate specificity of cytochrome P4
## Abstract For Abstract see ChemInform Abstract in Full Text.
## Abstract Directed evolution has become the preferred engineering approach to generate tailorβmade enzymes. The method follows the design guidelines of nature: Darwinian selection of genetic variants. This review discusses the different stages of directed evolution experiments with the focus on d