✦ LIBER ✦

Directed evolution of enzymes: Library screening strategies

✍ Scribed by Hans Leemhuis; Ronan M. Kelly; Lubbert Dijkhuizen

Publisher: John Wiley and Sons
Year: 2009
Tongue: English
Weight: 226 KB
Volume: 61
Category: Article
ISSN: 1521-6543
DOI: 10.1002/iub.165

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Directed evolution has become the preferred engineering approach to generate tailor‐made enzymes. The method follows the design guidelines of nature: Darwinian selection of genetic variants. This review discusses the different stages of directed evolution experiments with the focus on developments in screening and selection procedures. © 2009 IUBMB IUBMB Life 61(3): 222–228, 2009

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