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Direct somatic embryogenesis and plantlet regeneration from cotyledonary leaves of safflower

✍ Scribed by A. K. A. Mandal; A. K. Chatterji; S. Dutta Gupta

Publisher
Springer
Year
1995
Tongue
English
Weight
395 KB
Volume
43
Category
Article
ISSN
0167-6857

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✦ Synopsis

Somatic embryos were induced directly on adaxial surface of cotyledonary leaves within 8-10 days of culture on Murashige and Skoog medium containing 5.37 to 10.74 laM 1 -napthaleneacetic acid and 2.22 p.M benzyl adenine. Germinated embryos with shoot axes developed into complete plants after transfer onto half stength Murashige and Skoog medium containing 1.07 p.M 1 -napthaleneacetic acid. Histological studies suggested direct origin of somatic embryos with broad-base attachment.

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