✦ LIBER ✦

Direct selection of recombinant plasmids with chlorate

✍ Scribed by Jochen Reiss; Walter Klingmüller

Book ID: 109313763
Publisher: John Wiley and Sons
Year: 1987
Tongue: English
Weight: 383 KB
Volume: 43
Category: Article
ISSN: 0378-1097
DOI: 10.1111/j.1574-6968.1987.tb02123.x

No coin nor oath required. For personal study only.

📜 SIMILAR VOLUMES

Marker rescue allows direct selection fo

Marker rescue allows direct selection for recombinant plasmids in streptococci

✍ Behnke, Detlev 📂 Article 📅 1982 🏛 Springer 🌐 English ⚖ 276 KB

Resident deletion derivatives (Ems or Cms) of the streptococcal plasmid vector pGB301 rescue antibiotic resistance genes from linearized pGB301 (Emr, Cmr) DNA with high frequency. Insertion of passenger DNA next to an antibiotic resistance determinant of pGB301, which is missing on the resident plas

Construction of an HpaI and HindII plasm

Construction of an HpaI and HindII plasmid vector allowing direct selection of transformants harboring recombinant plasmids

✍ Schumann, Wolfgang 📂 Article 📅 1979 🏛 Springer 🌐 English ⚖ 374 KB

The construction of the vector plasmid PKN80 is described, which can be used as HpaI or HindII cloning vehicle with direct selection on transformants harboring hybrid plasmids. pKN80 carries the EcoRI.C fragment of phage Mu DNA coding for a killing function which is efficiently expressed upon transf

Novel plasmid marker rescue transformati

Novel plasmid marker rescue transformation system for molecular cloning in Bacillus subtilis enabling direct selection of recombinants

✍ Haima, Peter ;Bron, Sierd ;Venema, Gerard 📂 Article 📅 1990 🏛 Springer 🌐 English ⚖ 808 KB

A versatile plasmid marker rescue transformation system was developed for homology-facilitated cloning in Bacillus subtilis. It is based on the highly efficient host-vector system 6GM15-pHPS9, which allows the direct selection of recombinants by means of beta-galactosidase alpha-complementation. The

Construction and properties of a new clo

Construction and properties of a new cloning vehicle, allowing direct sereening for recombinant plasmids

✍ Rüther, Ulrich 📂 Article 📅 1980 🏛 Springer 🌐 English ⚖ 221 KB

pUR2, a certified B2(EK2) vector, allows easy isolation of variants containing cloned EcoRI-fragments. Bacteria harboring plasmids without inserts make blue colonies on indicator-plates, whilst those harboring recombinant plasmids make white colonies.

Selective transduction of recombinant pl

Selective transduction of recombinant plasmids with cloned pac sites by Salmonella phage P22

✍ Schmidt, Christine ;Schmieger, Horst 📂 Article 📅 1984 🏛 Springer 🌐 English ⚖ 620 KB

Nif-hybrids of enterobacter: Selection f

Nif-hybrids of enterobacter: Selection for nif gene integration with chlorate

✍ Klingmüller, W. ;Shanmugam, K. T. ;Singh, M. 📂 Article 📅 1983 🏛 Springer 🌐 English ⚖ 744 KB

The nif gene group from Klebsiella can be transferred into Enterobacter cloacae by conjugation using Escherichia coli donor cells carrying the composite self-transmissible nif-plasmid pRD1. A small fraction of the hybrids obtained is stable upon prolonged passaging without selection. Their stability