✦ LIBER ✦

Marker rescue allows direct selection for recombinant plasmids in streptococci

✍ Scribed by Behnke, Detlev

Book ID: 104726596
Publisher: Springer
Year: 1982
Tongue: English
Weight: 276 KB
Volume: 188
Category: Article
ISSN: 0026-8925
DOI: 10.1007/bf00333012

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✦ Synopsis

Resident deletion derivatives (Ems or Cms) of the streptococcal plasmid vector pGB301 rescue antibiotic resistance genes from linearized pGB301 (Emr, Cmr) DNA with high frequency. Insertion of passenger DNA next to an antibiotic resistance determinant of pGB301, which is missing on the resident plasmid, forces corescue of these two plasmid domains, thus allowing direct selection for recombinant plasmids.

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