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Direct reverse transcription-polymerase chain reaction from whole blood without RNA extraction

✍ Scribed by Yi-Jun Shi; Jing-Zhong Liu

Book ID
113409565
Publisher
Elsevier Science
Year
1992
Tongue
English
Weight
279 KB
Volume
9
Category
Article
ISSN
1050-3862

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Highly sensitive detection of viral RNA
Highly sensitive detection of viral RNA genomes in blood specimens by an optimized reverse transcription-polymerase chain reaction
✍ Shigeki Murakami; Yoshiyuki Takahashi; Shigeru Yoshida; Isao Fuke; Kozo Ohmae; C 📂 Article 📅 1994 🏛 John Wiley and Sons 🌐 English ⚖ 722 KB

## Abstract A protocol was developed for a highly sensitive detection of viral RNA in blood specimens by reverse transcription coupled with a nested poly‐merase chain reaction. Using Japanese encephalitis virus (JEV) as a model, the optimized reverse transcription‐polymerase chain reaction (ORTPCR)