Direct fluorometric analysis of aromatic polycyclic hydrocarbons on thin layer chromatograms
✍ Scribed by René Tomingas; Gisela Voltmer; Roman Bednarik
- Book ID
- 119028317
- Publisher
- Elsevier Science
- Year
- 1977
- Tongue
- English
- Weight
- 279 KB
- Volume
- 7
- Category
- Article
- ISSN
- 0048-9697
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A procedure which combines thin-layer chromatography with position-sensing proportional counting has been developed for analyzing the metabolism of carcinogenic polycyclic aromatic hydrocarbons. The profiles of the metabolites of [3H]benzo(a)pyrene and 7,12-[12-~4C]dimethylbenz(a)anthracene produced
in an air oven at XOO--ILOO for 30-40 min. The compounds in (100-200 pg) were spotted on the glass plates in the usual way. ## 571 chloroform solution The solvent was allowed to run up to a height of 14-15 cm from the base line. The plates were then air-dried, sprayed with a solution of acetic anh