Analysis of metabolism of carcinogenic polycyclic hydrocarbons by position-sensing proportional counting of thin-layer chromatograms

✍ Scribed by William M. Baird; Leila Diamond; Thaddeus W. Borun; Seth Shulman

• Book ID: 102627082
• Publisher: Elsevier Science
• Year: 1979
• Tongue: English
• Weight: 399 KB
• Volume: 99
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(79)90058-7

✦ Synopsis

A procedure which combines thin-layer chromatography with position-sensing proportional counting has been developed for analyzing the metabolism of carcinogenic polycyclic aromatic hydrocarbons. The profiles of the metabolites of [3H]benzo(a)pyrene and 7,12-[12-~4C]dimethylbenz(a)anthracene produced in cell culture were comparable when obtained by this procedure and by standard methods. However, position-sensing proportional countingallows simultaneous counting of all components of a sample within lo-20 min, and thereby permits the analysis of many hydrocarbon samples in a short time. In addition, the procedure eliminates the necessity of cutting or scraping carcinogen-containing thin-layer chromatograms.