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Direct Enantiomeric Separation of Cathinone and One Major Metabolite on Cellobiohydrolase (CBH-I) Chiral Stationary Phase

✍ Scribed by Hassan Y. Aboul-Enein; Vince Serignese

Publisher
John Wiley and Sons
Year
1997
Tongue
English
Weight
157 KB
Volume
11
Category
Article
ISSN
0269-3879

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✦ Synopsis

A direct, isocratic high-performance liquid chromatographic (HPLC) method is described for the enantiomeric separation of racemic cathinone and one of its metabolites, (+I-)-norephedrine, on a protein-based chiral stationary phase (CSP), cellobiohydrolase, known as CBH-I, under reversed-phase conditions. It was determined that the S-(-)-enantiomer eluted first for cathinone whiie the 1S,2R-(+)-enantiomer eluted first for norephedrine. The procedure is simple with no sample derivatization required and a short assay time.

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