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Dimerization of Double-Stranded RNA Possessing a 3′-Overhanging Single-Stranded End

✍ Scribed by Vogtherr, Martin; Kirchner, Roland; Zuleeg, Tilman; Limmer, Stefan

Book ID
127318732
Publisher
Adenine Press
Year
1999
Tongue
English
Weight
833 KB
Volume
17
Category
Article
ISSN
0739-1102

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A simple method for separation of ssRNA from dsRNA on hydroxyapatite is described. The following procedure can be usefully applied for RNA fractionation: (i) Elution of column with 0.1 M phosphate at 60" removes nueleotidrs and a sm:ilI amount of ssRNA. (ii) Elution with 0.1 31 phosphate at 90" remo