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Purification and properties of a novel nucleolar exoribonuclease capable of degrading both single-stranded and double-stranded RNA

✍ Scribed by Eichler, Duane C.; Eales, Susan J.


Book ID
126921857
Publisher
American Chemical Society
Year
1985
Tongue
English
Weight
705 KB
Volume
24
Category
Article
ISSN
0006-2960

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A simple method for separation of ssRNA from dsRNA on hydroxyapatite is described. The following procedure can be usefully applied for RNA fractionation: (i) Elution of column with 0.1 M phosphate at 60" removes nueleotidrs and a sm:ilI amount of ssRNA. (ii) Elution with 0.1 31 phosphate at 90" remo