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A simple method for the separation of single-stranded and double-stranded RNA on hydroxyapatite

✍ Scribed by J. Kalmakoff; C.C. Payne

Publisher: Elsevier Science
Year: 1973
Tongue: English
Weight: 832 KB
Volume: 55
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(73)90287-x

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✦ Synopsis

A simple method for separation of ssRNA from dsRNA on hydroxyapatite is described. The following procedure can be usefully applied for RNA fractionation: (i) Elution of column with 0.1 M phosphate at 60" removes nueleotidrs and a sm:ilI amount of ssRNA. (ii) Elution with 0.1 31 phosphate at 90" removes all the wmaining ssRNA. (iii) Finally, elution with 0.3 M phosphate at 90" removes the double-stranded RNA. This stepwise elution using a combination of phosphate concentration and temperature gives 96-100% recovery of the mntcrinl applied.

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