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A method for measuring the number of single- and double-strand breaks introduced into DNA molecules by the action of deoxyribonucleases

✍ Scribed by Angeline S. Douvas

Publisher: Elsevier Science
Year: 1978
Tongue: English
Weight: 731 KB
Volume: 90
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(78)90013-1

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✦ Synopsis

A method is described for measuring the average number of nucleaseinduced single-and double-strand breaks per DNA molecule. The procedure involves measuring the weight-average molecular weight of DNase I-digested DNA under neutral and alkaline conditions. A statistical equation is used to calculate

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