Diffusion in polyacrylamide gels
β Scribed by W. Brown; R.M. Johnsen
- Publisher
- Elsevier Science
- Year
- 1981
- Tongue
- English
- Weight
- 442 KB
- Volume
- 22
- Category
- Article
- ISSN
- 0032-3861
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π SIMILAR VOLUMES
Swelling behavior of polyacrylamide (PAAm) and polyacrylamide-co-polyacrylic acid (PAAm-co-PAAc) gels was investigated in aqueous solutions of monodisperse PAAms with molecular weights ( M V w ) ranging from 1.5 1 10 3 to 5 1 10 6 g/mol. The volume of the gels decreases as the PAAm concentration in
## Polyacrylamide gels may be destained either electrophoretically (e.g., 1, 2) or by simple diffusion destaining in which gels are soaked in media (most commonly) of 7.5% acetic acid (3-5). Maurer ( 6) gives a concise appraisal of the advantages and disadvantages of both methods. Electrophoretic
The weight conversion at the gel point (~) was determined by the gas bubble method in two sets of polyacrylamide (PAA) gels crosslinked with N,N'-methylene-bisacrylamide (BA). ~t~ remained practically constant when the crosslinker ratio was C = 10% (w/w) and the total concentration of comonomers in
The structure of polyacrylamide gels was studied using proton spin-lattice relaxation and PFG diffusion methods. Polyacrylamide gels, with total polymer concentrations ranging from 0.25 to 0.35 g/ml and crosslinker concentrations from 0 to 10% by weight, were studied. The data showed no effect of th