Transient state isoelectric focusing: Experimental determination of apparent diffusion coefficients in polyacrylamide gels
β Scribed by Nicholas Catsimpoolas
- Publisher
- Elsevier Science
- Year
- 1973
- Tongue
- English
- Weight
- 440 KB
- Volume
- 54
- Category
- Article
- ISSN
- 0003-2697
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The continuous scanning apparatus developed by Catsimpoolas was applied to an analysis of the concentration profiles of a protein, ~-la~to~lobulin B, while it was subjected to polyacrylamide gel electrophoresis (PAGE) in a multiphasic buffer system. Continuous optical scanning in PAGE permitted reli
The first step toward protein structure determination using of self-diffusion coefficient in peptides (17,18), proteins NMR is elucidation of the appropriate solution conditions (9-14), and DNA oligomers (15). Altieri et al. (12) have that obviate any protein self-association at the millimolar recen