Simple device for continuous diffusion destaining of polyacrylamide gels
โ Scribed by Lawrence J. Gathercole; LeRoy Klein
- Publisher
- Elsevier Science
- Year
- 1971
- Tongue
- English
- Weight
- 174 KB
- Volume
- 44
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
โฆ Synopsis
Polyacrylamide
gels may be destained either electrophoretically (e.g., 1, 2) or by simple diffusion destaining in which gels are soaked in media (most commonly) of 7.5% acetic acid (3-5).
Maurer ( 6) gives a concise appraisal of the advantages and disadvantages of both methods. Electrophoretic
๐ SIMILAR VOLUMES
dling and storage. The overall quality ofthe second-dimension gel is improved by eliminating the dark carrier ampholyte streak at the front of the basic end of the gel as well as the SDS-Triton artifact at the acidic end. The degree of protein resolution is essentially identical for the two techniqu
## A method that is both simple and inexpensive is described for preparative polyacrylamide gel electrophoresis of low-molecular-weight RNA. The utility of the method is demonstrated by the preparative separation of 4S and 5s RX-i.