The thiamine diphosphate-dependent enzyme 1-deoxy-D-xylulose 5-phosphate synthase from E. coli can use D-erythrose 4-phosphate and D-ribose 5-phosphate as alternative substrates. These reactions were used for the production of 1-deoxy-D-fructose 6-phosphate and 1-deoxy-D-sedoheptulose 7-phosphate an
Differential expression of three 1-deoxy-D-xylulose-5-phosphate synthase genes in rice
β Scribed by Bo-Ra Kim; Soo-Un Kim; Yung-Jin Chang
- Publisher
- Springer Netherlands
- Year
- 2005
- Tongue
- English
- Weight
- 190 KB
- Volume
- 27
- Category
- Article
- ISSN
- 0141-5492
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We report a novel fluorometric end-point assay for the determination of 1-deoxy-D-xylulose 5-phosphate synthase (DXS) activity based on the reaction of 1-deoxy-D-xylulose 5-phosphate (DX5P) with 3,5-diaminobenzoic acid in an acidic medium to form a highly fluorescent quinaldine derivative. The assay
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