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Enzymatic synthesis of 1-deoxysugar-phosphates using E. coli 1-deoxy-d-xylulose 5-phosphate synthase

✍ Scribed by Jordi Querol; Catherine Grosdemange-Billiard; Michel Rohmer; Albert Boronat; Santiago Imperial

Publisher
Elsevier Science
Year
2002
Tongue
French
Weight
96 KB
Volume
43
Category
Article
ISSN
0040-4039

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✦ Synopsis

The thiamine diphosphate-dependent enzyme 1-deoxy-D-xylulose 5-phosphate synthase from E. coli can use D-erythrose 4-phosphate and D-ribose 5-phosphate as alternative substrates. These reactions were used for the production of 1-deoxy-D-fructose 6-phosphate and 1-deoxy-D-sedoheptulose 7-phosphate and have potential application for the biosynthesis of other 1-deoxysugar phosphates.

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## Abstract D‐Glyceraldehyde 3‐phosphate (=D‐GAP; **2**) was prepared by an improved chemical method (__Schemeβ€…2__), and it was then employed to synthesize 1‐deoxy‐D‐xylulose 5‐phosphate (=DXP; **3**) which is enzymatically one of the key intermediates in the MEP (**4**) terpenoid biosynthetic path