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Direct formation of 2-C-methyl-d-erythritol 4-phosphate from 1-deoxy-d-xylulose 5-phosphate by 1-deoxy-d-xylulose 5-phosphate reductoisomerase, a new enzyme in the non-mevalonate pathway to isopentenyl diphosphate

✍ Scribed by Tomohisa Kuzuyama; Shunji Takahashi; Hiroyuki Watanabe; Haruo Seto

Publisher
Elsevier Science
Year
1998
Tongue
French
Weight
260 KB
Volume
39
Category
Article
ISSN
0040-4039

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✦ Synopsis

1-Deoxy-D-xyluiose 5-phosphate is biotransformed to 2-C-methyl-D-erythritol 4-phosphate in a single step in the presence of NADPH by a new recombinant enzyme named l-deoxy-D-xylulose 5-phosphate reductoisomerase purified from Escherichia coli.

πŸ“œ SIMILAR VOLUMES

Elucidation of the 2-C-methyl-d-erythrit
Elucidation of the 2-C-methyl-d-erythritol 4-phosphate pathway for isoprenoid biosynthesis: straightforward syntheses of enantiopure 1-deoxy-d-xylulose from pentose derivatives
✍ Jean-FranΓ§ois Hoeffler; Catherine Grosdemange-Billiard; Michel Rohmer πŸ“‚ Article πŸ“… 2001 πŸ› Elsevier Science 🌐 French βš– 65 KB

Optically pure 1-deoxy-D-xylulose, a key metabolite for feeding experiments in the methylerythritol phosphate pathway for isoprenoid biosynthesis, is conveniently synthesised from 1,2-O-isopropylidene-a-D-xylofuranose or from D-arabinose. This renders labelling with hydrogen isotopes possible.