Differences in protein staining by Coomassie Brilliant Blue and neutron activated Coomassie Brilliant Blue dyes

✍ Scribed by Dr. Douglas M. Gersten; Pamela H. Wolf; Edward J. Zapolski

• Book ID: 102831638
• Publisher: John Wiley and Sons
• Year: 1987
• Tongue: English
• Weight: 634 KB
• Volume: 8
• Category: Article
• ISSN: 0173-0835
• DOI: 10.1002/elps.1150081202

✦ Synopsis

Differences in protein staining by Coomassie Brilliant Blue and neutron activated Coomassie Brilliant Blue dyes

The degree of interaction between protein and stains depends in large part upon the protein's content of basic amino acids. We have compared "staining" characteristics of Coomassie Brilliant Blue R-250 with a dye that was rendered radioactive by neutron bombardment. The protein-dye intensities were compared to amino acid composition as in our previous study (Electrophoresis 1986,7,327-332). While the data for "colored" dye reflected involvement of basic amino acid residues, quite unexpectedly, the radioactive dye appeared to act in part with the acidic amino acids. This may be due to enhanced binding of dye impurities or radiolysis products.